Device

Part:BBa_M36598:Design

Designed by: Daniel Hu, Andrew Austin Lee, Clinton Olivas   Group: Stanford BIOE44 - S11   (2014-10-24)


Yeast-Optimized Chicken Lysozyme (LysC) Secretor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 989
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We checked to make sure the sequence did not include any BsaI restriction sites because we are cloning the gene into a vector using BsaI. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized.


Source

The Lysozyme sequence is originally derived from Gallus Gallus. The Gal1 promoter and Cyc1 terminator are originally derived from Saccharomyces cerevisiae.

References